The new developed real-time PCR screening- and identification kit detects and identifies 30 relevant beer spoilage microorgansims. The simultaneously detection of the Enterobacteriaceae group and the spoilage yeasts Saccharomyces diastaticus and Pichia anomala are unique. The kit is ready-to-use that means all relevant primers and probes, including inhibition- and positive control DNA are freeze-dried. Only sample-DNA and Premix have to be added. Therefore the contamination risk is reduced and the handling is very easy. The evaluation is done in 3 detection channels (FAM,HEX,ROX) and the kit has an open platform for several real-time PCR machines. In table 1 the detected and identified microorganisms are presented.